miRNA: 3'- aGUAGGUGGA-CUUgUCugGCGa -5'
miRNA: 3'- agUAGGUGGACUUGUCu----GGCGA- -5'
miRNA: 3'- -AGUAGGUGGACuUGU-CUGGCGa -5'
miRNA: 3'- aGUAGGUGGACu--UGUCUGGCga -5'
miRNA: 3'- -AGUAGGUGGACUUGUCUGGCGA- -5'
Results 41 - 45 of 45 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | - | 235059 | 0.72 | 0.941132 |
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Target: 5'- gCGUCCACCUaGGAgGGugCGCg -3' miRNA: 3'- aGUAGGUGGA-CUUgUCugGCGa -5' |
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| MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | - | 35719 | 0.72 | 0.930619 |
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Target: 5'- -gAUCCGCCUGGGCGGcucgcCCGCUu -3' miRNA: 3'- agUAGGUGGACUUGUCu----GGCGA- -5' |
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| MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | + | 59382 | 0.74 | 0.885195 |
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Target: 5'- cUCAUCCACUUGcACAuGGCCGCc -3' miRNA: 3'- -AGUAGGUGGACuUGU-CUGGCGa -5' |
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| MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | + | 22401 | 0.75 | 0.827258 |
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Target: 5'- aCGUCCGCCUGuacGCGGACCGa- -3' miRNA: 3'- aGUAGGUGGACu--UGUCUGGCga -5' |
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| MIMAT0001582 | hcmv-miR-US25-2-5p | -48.6 | NC_006273.1 | + | 221516 | 1.01 | 0.039854 |
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Target: 5'- cUCAUCCACCUGAACAGACCGCUa -3' miRNA: 3'- -AGUAGGUGGACUUGUCUGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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