Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10634 | 5' | -55.3 | NC_002702.1 | + | 12615 | 0.66 | 0.593157 |
Target: 5'- cGUGGcCAccuaugauugggUGACGCuGAGCauaCCGGAGGGc -3' miRNA: 3'- cCGCCaGU------------ACUGCG-CUUG---GGCCUUCC- -5' |
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10634 | 5' | -55.3 | NC_002702.1 | + | 21026 | 0.67 | 0.537988 |
Target: 5'- uGGuUGGUCGUcGAUGaCGAGCCUGGGc-- -3' miRNA: 3'- -CC-GCCAGUA-CUGC-GCUUGGGCCUucc -5' |
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10634 | 5' | -55.3 | NC_002702.1 | + | 7996 | 0.69 | 0.434043 |
Target: 5'- uGGCgcaGGUCGUGAUGCGGGCCa-GAGa- -3' miRNA: 3'- -CCG---CCAGUACUGCGCUUGGgcCUUcc -5' |
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10634 | 5' | -55.3 | NC_002702.1 | + | 15474 | 0.71 | 0.318159 |
Target: 5'- uGCgGGUCGacacuggGACGCGAACCCGauAGGu -3' miRNA: 3'- cCG-CCAGUa------CUGCGCUUGGGCcuUCC- -5' |
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10634 | 5' | -55.3 | NC_002702.1 | + | 12636 | 1.13 | 0.000345 |
Target: 5'- uGGCGGUCAUGACGCGAACCCGGAAGGa -3' miRNA: 3'- -CCGCCAGUACUGCGCUUGGGCCUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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