Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10681 | 5' | -51 | NC_002730.1 | + | 30485 | 0.66 | 0.818783 |
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Target: 5'- uAUGUGUUUA--AGCAGUCacaGGCGGCc -3' miRNA: 3'- -UACGCAAGUagUCGUUAGg--CCGUUGu -5' |
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| 10681 | 5' | -51 | NC_002730.1 | + | 20940 | 0.66 | 0.787841 |
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Target: 5'- uUGaUGUcCAUCAGCAGaccUUCGGCAAUg -3' miRNA: 3'- uAC-GCAaGUAGUCGUU---AGGCCGUUGu -5' |
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| 10681 | 5' | -51 | NC_002730.1 | + | 12799 | 0.67 | 0.766249 |
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Target: 5'- --uUGUUgAUCAGCGGUCaugcaguGGCAACAa -3' miRNA: 3'- uacGCAAgUAGUCGUUAGg------CCGUUGU- -5' |
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| 10681 | 5' | -51 | NC_002730.1 | + | 27251 | 0.67 | 0.755204 |
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Target: 5'- -gGCGUUUugCAGUAAgucCCGGCAAUg -3' miRNA: 3'- uaCGCAAGuaGUCGUUa--GGCCGUUGu -5' |
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| 10681 | 5' | -51 | NC_002730.1 | + | 28736 | 0.69 | 0.615339 |
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Target: 5'- -aGCGUgCA-CGGaCAGUUCGGCGGCAu -3' miRNA: 3'- uaCGCAaGUaGUC-GUUAGGCCGUUGU- -5' |
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| 10681 | 5' | -51 | NC_002730.1 | + | 27227 | 1.07 | 0.001821 |
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Target: 5'- uAUGCGUUCAUCAGCAAUCCGGCAACAg -3' miRNA: 3'- -UACGCAAGUAGUCGUUAGGCCGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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