Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11300 | 3' | -50.9 | NC_003083.1 | + | 9324 | 0.72 | 0.762573 |
Target: 5'- uGAGCGccgaaACGAUUGCGCGCGCGa--- -3' miRNA: 3'- -UUCGUug---UGCUGAUGCGCGUGCaaua -5' |
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11300 | 3' | -50.9 | NC_003083.1 | + | 80126 | 0.73 | 0.699376 |
Target: 5'- gAGGCAACGCGugcGCGCGCAUGUc-- -3' miRNA: 3'- -UUCGUUGUGCugaUGCGCGUGCAaua -5' |
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11300 | 3' | -50.9 | NC_003083.1 | + | 22268 | 1.04 | 0.011298 |
Target: 5'- uAAGCAACACGACUACGCGCACGUUAUa -3' miRNA: 3'- -UUCGUUGUGCUGAUGCGCGUGCAAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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