Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11623 | 3' | -52.8 | NC_003092.1 | + | 4698 | 0.67 | 0.359568 |
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Target: 5'- --uGACCGCGacgcacguCUGCgGCAACAacaaAGCc -3' miRNA: 3'- guuCUGGCGCgu------GACGaCGUUGU----UCG- -5' |
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| 11623 | 3' | -52.8 | NC_003092.1 | + | 1883 | 0.69 | 0.280125 |
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Target: 5'- gCGGGuACCGCa-GCUGCaucaGCGACAGGCu -3' miRNA: 3'- -GUUC-UGGCGcgUGACGa---CGUUGUUCG- -5' |
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| 11623 | 3' | -52.8 | NC_003092.1 | + | 2571 | 0.7 | 0.264483 |
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Target: 5'- gGGGA-CGCGC-UUGUUGCAGCAGuGCg -3' miRNA: 3'- gUUCUgGCGCGuGACGACGUUGUU-CG- -5' |
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| 11623 | 3' | -52.8 | NC_003092.1 | + | 1378 | 0.71 | 0.221796 |
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Target: 5'- uGAGGCCcaGCGCGCUauccucGC-GCAACAGGUc -3' miRNA: 3'- gUUCUGG--CGCGUGA------CGaCGUUGUUCG- -5' |
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| 11623 | 3' | -52.8 | NC_003092.1 | + | 2372 | 0.75 | 0.105392 |
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Target: 5'- uCGAGGCCuGCGCGCaugGCUGguGCucuAGCu -3' miRNA: 3'- -GUUCUGG-CGCGUGa--CGACguUGu--UCG- -5' |
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| 11623 | 3' | -52.8 | NC_003092.1 | + | 2522 | 1.13 | 0.000138 |
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Target: 5'- cCAAGACCGCGCACUGCUGCAACAAGCg -3' miRNA: 3'- -GUUCUGGCGCGUGACGACGUUGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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