Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11852 | 5' | -65.2 | NC_003266.2 | + | 15959 | 0.66 | 0.204295 |
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Target: 5'- cGuGcCCGuGGCaCC-CGCCCCCcUCGCAc -3' miRNA: 3'- cCuC-GGC-CCGaGGaGCGGGGG-AGCGU- -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 865 | 0.66 | 0.199102 |
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Target: 5'- uGAGCUaGGGCUUgaaGCCUCCUCgGCAg -3' miRNA: 3'- cCUCGG-CCCGAGgagCGGGGGAG-CGU- -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 22254 | 0.66 | 0.194024 |
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Target: 5'- --uGCUGGGC-CUUgGCUCCCUCGg- -3' miRNA: 3'- ccuCGGCCCGaGGAgCGGGGGAGCgu -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 8851 | 0.66 | 0.189059 |
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Target: 5'- cGAGgCGGGCaugaaCGCCUUCUCGCAg -3' miRNA: 3'- cCUCgGCCCGagga-GCGGGGGAGCGU- -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 20248 | 0.66 | 0.189059 |
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Target: 5'- --cGCUGGGCUCCggguUCGaCCCCUacuUCGUc -3' miRNA: 3'- ccuCGGCCCGAGG----AGC-GGGGG---AGCGu -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 16593 | 0.67 | 0.170306 |
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Target: 5'- -uGGUgGGGUacacaUCCUCGCCCUCgCGCu -3' miRNA: 3'- ccUCGgCCCG-----AGGAGCGGGGGaGCGu -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 23669 | 0.67 | 0.165448 |
|
Target: 5'- cGGGCCagaugcuugaugaGGGCgUCCUC-CCCCC-CGCu -3' miRNA: 3'- cCUCGG-------------CCCG-AGGAGcGGGGGaGCGu -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 9700 | 0.67 | 0.163281 |
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Target: 5'- -aAGCCGGGCccaaagaccuggugcUCCUCGCCaCCaucCGCc -3' miRNA: 3'- ccUCGGCCCG---------------AGGAGCGG-GGga-GCGu -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 5450 | 0.68 | 0.145291 |
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Target: 5'- cGAGuCCGucUUCCUCGCCCCCaaGCu -3' miRNA: 3'- cCUC-GGCccGAGGAGCGGGGGagCGu -5' |
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| 11852 | 5' | -65.2 | NC_003266.2 | + | 6724 | 1.09 | 0.000064 |
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Target: 5'- cGGAGCCGGGCUCCUCGCCCCCUCGCAc -3' miRNA: 3'- -CCUCGGCCCGAGGAGCGGGGGAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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