miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12323 3' -49.5 NC_003324.1 + 34196 0.66 0.945696
Target:  5'- cGACAGg--GAGgCGUGAGUGCgCGAGu -3'
miRNA:   3'- -UUGUCagaCUCaGUACUCACG-GUUUg -5'
12323 3' -49.5 NC_003324.1 + 12210 0.68 0.845287
Target:  5'- gGACGGUCUcgcGGGUCucgcaGAGaUGCCGAAUg -3'
miRNA:   3'- -UUGUCAGA---CUCAGua---CUC-ACGGUUUG- -5'
12323 3' -49.5 NC_003324.1 + 3935 0.72 0.650991
Target:  5'- gAGCAGUgaCUGcG-CAUGAGUGCUGAACa -3'
miRNA:   3'- -UUGUCA--GACuCaGUACUCACGGUUUG- -5'
12323 3' -49.5 NC_003324.1 + 4058 1.08 0.003769
Target:  5'- cAACAGUCUGAGUCAUGAGUGCCAAACu -3'
miRNA:   3'- -UUGUCAGACUCAGUACUCACGGUUUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.