Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12506 | 5' | -61.4 | NC_003345.1 | + | 33062 | 0.66 | 0.50455 |
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Target: 5'- -aGGGcuacgccGGGCCGACCGCCaacccgCCGGa---- -3' miRNA: 3'- agUCC-------CCCGGCUGGCGG------GGCCaauug -5' |
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| 12506 | 5' | -61.4 | NC_003345.1 | + | 29805 | 0.69 | 0.346317 |
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Target: 5'- cCGuGGGG-CGACCGCCUCGGUc--- -3' miRNA: 3'- aGUcCCCCgGCUGGCGGGGCCAauug -5' |
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| 12506 | 5' | -61.4 | NC_003345.1 | + | 286 | 0.71 | 0.231975 |
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Target: 5'- gCA-GGGGCCGACCGgCCCGagaGACg -3' miRNA: 3'- aGUcCCCCGGCUGGCgGGGCcaaUUG- -5' |
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| 12506 | 5' | -61.4 | NC_003345.1 | + | 77573 | 0.71 | 0.231975 |
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Target: 5'- gCA-GGGGCCGACCGgCCCGagaGACg -3' miRNA: 3'- aGUcCCCCGGCUGGCgGGGCcaaUUG- -5' |
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| 12506 | 5' | -61.4 | NC_003345.1 | + | 75301 | 0.73 | 0.185405 |
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Target: 5'- gCAGGGcugaaccGCCGACCucgcgGCCCCGGUUAc- -3' miRNA: 3'- aGUCCCc------CGGCUGG-----CGGGGCCAAUug -5' |
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| 12506 | 5' | -61.4 | NC_003345.1 | + | 688 | 1.1 | 0.000373 |
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Target: 5'- uUCAGGGGGCCGACCGCCCCGGUUAACa -3' miRNA: 3'- -AGUCCCCCGGCUGGCGGGGCCAAUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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