Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12518 | 3' | -52.5 | NC_003345.1 | + | 23186 | 0.69 | 0.759163 |
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Target: 5'- aGCGCC---ACCGAC-CGGCAGAAGu- -3' miRNA: 3'- -UGCGGcagUGGUUGaGCCGUCUUUug -5' |
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| 12518 | 3' | -52.5 | NC_003345.1 | + | 45285 | 0.7 | 0.706442 |
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Target: 5'- --aCCGUCACCAcACUCcgGGCAGGuGAGCg -3' miRNA: 3'- ugcGGCAGUGGU-UGAG--CCGUCU-UUUG- -5' |
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| 12518 | 3' | -52.5 | NC_003345.1 | + | 60183 | 0.71 | 0.684763 |
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Target: 5'- aACGCCG-CACCAGCacCGGCcauAGcGAGCa -3' miRNA: 3'- -UGCGGCaGUGGUUGa-GCCG---UCuUUUG- -5' |
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| 12518 | 3' | -52.5 | NC_003345.1 | + | 25409 | 0.71 | 0.684763 |
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Target: 5'- aGCGCCGUCccgcucuCCGAgggCGGCgaAGAAGACg -3' miRNA: 3'- -UGCGGCAGu------GGUUga-GCCG--UCUUUUG- -5' |
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| 12518 | 3' | -52.5 | NC_003345.1 | + | 21637 | 0.71 | 0.640887 |
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Target: 5'- uCGCCGguaucacuaUCGCCAACUCgGGCGGc-GACg -3' miRNA: 3'- uGCGGC---------AGUGGUUGAG-CCGUCuuUUG- -5' |
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| 12518 | 3' | -52.5 | NC_003345.1 | + | 23242 | 0.77 | 0.357778 |
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Target: 5'- uCGCCGUCuCC-GCUCGGUcggGGAGAGCg -3' miRNA: 3'- uGCGGCAGuGGuUGAGCCG---UCUUUUG- -5' |
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| 12518 | 3' | -52.5 | NC_003345.1 | + | 12768 | 1.09 | 0.002564 |
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Target: 5'- aACGCCGUCACCAACUCGGCAGAAAACu -3' miRNA: 3'- -UGCGGCAGUGGUUGAGCCGUCUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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