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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12520 | 5' | -56.6 | NC_003345.1 | + | 861 | 0.69 | 0.567991 |
|
Target: 5'- -cCCGCCCCCgaccgUUCGGGggGagauuaGCGg -3' miRNA: 3'- aaGGUGGGGGa----AAGCCCuuCacc---UGC- -5' |
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| 12520 | 5' | -56.6 | NC_003345.1 | + | 6261 | 0.68 | 0.621195 |
|
Target: 5'- -cCUACUCCCga--GGGAAGUGGGa- -3' miRNA: 3'- aaGGUGGGGGaaagCCCUUCACCUgc -5' |
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| 12520 | 5' | -56.6 | NC_003345.1 | + | 12703 | 0.7 | 0.495736 |
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Target: 5'- -cUCGCUCCC-UUCGGGGaguugugaaagAGUGGAUGg -3' miRNA: 3'- aaGGUGGGGGaAAGCCCU-----------UCACCUGC- -5' |
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| 12520 | 5' | -56.6 | NC_003345.1 | + | 14530 | 1.07 | 0.00161 |
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Target: 5'- cUUCCACCCCCUUUCGGGAAGUGGACGa -3' miRNA: 3'- -AAGGUGGGGGAAAGCCCUUCACCUGC- -5' |
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| 12520 | 5' | -56.6 | NC_003345.1 | + | 43115 | 0.66 | 0.706283 |
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Target: 5'- aUCgACCUCCa--CGGGGAGcgGGACa -3' miRNA: 3'- aAGgUGGGGGaaaGCCCUUCa-CCUGc -5' |
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| 12520 | 5' | -56.6 | NC_003345.1 | + | 45016 | 0.66 | 0.737336 |
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Target: 5'- -aCCACagaCUCg-UCGGGGAGUGGAa- -3' miRNA: 3'- aaGGUGg--GGGaaAGCCCUUCACCUgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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