Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12548 | 5' | -59.9 | NC_003345.1 | + | 72217 | 0.66 | 0.605359 |
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Target: 5'- aUCGGCUCaacgaaagaCCGaccGAAaUCgUCGGUGGGg -3' miRNA: 3'- -AGCCGAG---------GGCa--CUUcAGgAGCCGCCC- -5' |
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| 12548 | 5' | -59.9 | NC_003345.1 | + | 7138 | 0.67 | 0.554488 |
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Target: 5'- --aGCUUCCGuUGGAaa-CUCGGCGGGa -3' miRNA: 3'- agcCGAGGGC-ACUUcagGAGCCGCCC- -5' |
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| 12548 | 5' | -59.9 | NC_003345.1 | + | 33840 | 0.67 | 0.554488 |
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Target: 5'- gUCGGCaUCCCGUa-----CUCGGCGGc -3' miRNA: 3'- -AGCCG-AGGGCAcuucagGAGCCGCCc -5' |
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| 12548 | 5' | -59.9 | NC_003345.1 | + | 25641 | 0.67 | 0.543448 |
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Target: 5'- cCGGCUCCgGUaucuaccGAGGUCa--GGCGuGGg -3' miRNA: 3'- aGCCGAGGgCA-------CUUCAGgagCCGC-CC- -5' |
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| 12548 | 5' | -59.9 | NC_003345.1 | + | 50333 | 0.67 | 0.514714 |
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Target: 5'- gCGGCUCCUucgGggGUCg-CGGUGGu -3' miRNA: 3'- aGCCGAGGGca-CuuCAGgaGCCGCCc -5' |
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| 12548 | 5' | -59.9 | NC_003345.1 | + | 18378 | 0.69 | 0.429171 |
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Target: 5'- -aGGCUCCCGUcuaauagagugagGAGGUCg-CGGUGGc -3' miRNA: 3'- agCCGAGGGCA-------------CUUCAGgaGCCGCCc -5' |
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| 12548 | 5' | -59.9 | NC_003345.1 | + | 70855 | 0.71 | 0.323597 |
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Target: 5'- aCGGCUCCauuacGGUCUUCGgGCGGGc -3' miRNA: 3'- aGCCGAGGgcacuUCAGGAGC-CGCCC- -5' |
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| 12548 | 5' | -59.9 | NC_003345.1 | + | 35189 | 1.11 | 0.000505 |
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Target: 5'- gUCGGCUCCCGUGAAGUCCUCGGCGGGg -3' miRNA: 3'- -AGCCGAGGGCACUUCAGGAGCCGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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