Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12551 | 3' | -50 | NC_003345.1 | + | 14677 | 0.66 | 0.969583 |
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Target: 5'- gUCGUGCUUcuccgcgacggcaaGCCGGACA--UAGCCuACc -3' miRNA: 3'- -AGCAUGAA--------------CGGCUUGUcuAUCGG-UGu -5' |
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| 12551 | 3' | -50 | NC_003345.1 | + | 75899 | 0.66 | 0.962599 |
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Target: 5'- gUCGUAUcgUGCCGGgucuuccuccuuggACGGccAGCCAUAg -3' miRNA: 3'- -AGCAUGa-ACGGCU--------------UGUCuaUCGGUGU- -5' |
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| 12551 | 3' | -50 | NC_003345.1 | + | 29133 | 0.67 | 0.951505 |
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Target: 5'- gUCGUagaagaagcACUUGCCGugaccguaguGACAGAgcgGGCCGg- -3' miRNA: 3'- -AGCA---------UGAACGGC----------UUGUCUa--UCGGUgu -5' |
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| 12551 | 3' | -50 | NC_003345.1 | + | 69648 | 0.67 | 0.94689 |
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Target: 5'- gUCGUggagGCUUGCCGAcCAGuacgagcGGUCGCGc -3' miRNA: 3'- -AGCA----UGAACGGCUuGUCua-----UCGGUGU- -5' |
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| 12551 | 3' | -50 | NC_003345.1 | + | 29940 | 0.68 | 0.925597 |
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Target: 5'- uUCGUG-UUGCCGAACGG--GGCgCACc -3' miRNA: 3'- -AGCAUgAACGGCUUGUCuaUCG-GUGu -5' |
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| 12551 | 3' | -50 | NC_003345.1 | + | 45950 | 0.69 | 0.877391 |
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Target: 5'- gUUGUGgUUGCgGAugGGGaAGCCACc -3' miRNA: 3'- -AGCAUgAACGgCUugUCUaUCGGUGu -5' |
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| 12551 | 3' | -50 | NC_003345.1 | + | 40372 | 0.7 | 0.861177 |
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Target: 5'- uUCGUACUUGUCGGGauucuucccgUAGuugccgAGCCACGu -3' miRNA: 3'- -AGCAUGAACGGCUU----------GUCua----UCGGUGU- -5' |
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| 12551 | 3' | -50 | NC_003345.1 | + | 55057 | 0.71 | 0.806862 |
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Target: 5'- cUCGUACaaGCCGGugGGuc-GCCACu -3' miRNA: 3'- -AGCAUGaaCGGCUugUCuauCGGUGu -5' |
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| 12551 | 3' | -50 | NC_003345.1 | + | 36754 | 1.05 | 0.009166 |
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Target: 5'- cUGUACUUGCCGAACAGAUAGCCACAa -3' miRNA: 3'- aGCAUGAACGGCUUGUCUAUCGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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