Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12562 | 5' | -58.7 | NC_003345.1 | + | 58372 | 0.66 | 0.571005 |
Target: 5'- --gGUGAaUAGCCUCUcCGCCGAGCu -3' miRNA: 3'- uugCGCUgAUUGGGGAcGCGGCUCGu -5' |
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12562 | 5' | -58.7 | NC_003345.1 | + | 19851 | 0.66 | 0.571005 |
Target: 5'- -cCGCGACUaccacGACCUCggugGgGCCGAuGCGg -3' miRNA: 3'- uuGCGCUGA-----UUGGGGa---CgCGGCU-CGU- -5' |
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12562 | 5' | -58.7 | NC_003345.1 | + | 63323 | 0.66 | 0.571005 |
Target: 5'- --aGCGAgCUGACUCCcgGCGCUG-GCGa -3' miRNA: 3'- uugCGCU-GAUUGGGGa-CGCGGCuCGU- -5' |
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12562 | 5' | -58.7 | NC_003345.1 | + | 27675 | 0.67 | 0.529408 |
Target: 5'- gAugGCGcuguucaaACUGGCCCgaGCGCaGGGCGg -3' miRNA: 3'- -UugCGC--------UGAUUGGGgaCGCGgCUCGU- -5' |
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12562 | 5' | -58.7 | NC_003345.1 | + | 69561 | 0.69 | 0.422004 |
Target: 5'- uGACGCaGACUGcUCCCUcgGCGUCGGGUg -3' miRNA: 3'- -UUGCG-CUGAUuGGGGA--CGCGGCUCGu -5' |
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12562 | 5' | -58.7 | NC_003345.1 | + | 46615 | 1.06 | 0.000966 |
Target: 5'- gAACGCGACUAACCCCUGCGCCGAGCAg -3' miRNA: 3'- -UUGCGCUGAUUGGGGACGCGGCUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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