Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1260 | 3' | -53.4 | NC_001317.1 | + | 27365 | 0.67 | 0.548115 |
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Target: 5'- uGGCGCGGCCGcUUGCGAcGCAuuGCGg--- -3' miRNA: 3'- -UCGUGCCGGU-AGUGUU-CGU--CGCaaua -5' |
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| 1260 | 3' | -53.4 | NC_001317.1 | + | 2951 | 0.67 | 0.548115 |
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Target: 5'- gGGCAUGGCC-UCGCAgaAGCAucuGCGc--- -3' miRNA: 3'- -UCGUGCCGGuAGUGU--UCGU---CGCaaua -5' |
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| 1260 | 3' | -53.4 | NC_001317.1 | + | 13371 | 0.67 | 0.536535 |
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Target: 5'- cAGCGCGGCg--CACAGGcCAGCa---- -3' miRNA: 3'- -UCGUGCCGguaGUGUUC-GUCGcaaua -5' |
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| 1260 | 3' | -53.4 | NC_001317.1 | + | 8573 | 0.69 | 0.426702 |
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Target: 5'- cGCACaG-CAUCAggcaGAGCAGCGUUAUa -3' miRNA: 3'- uCGUGcCgGUAGUg---UUCGUCGCAAUA- -5' |
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| 1260 | 3' | -53.4 | NC_001317.1 | + | 15681 | 0.69 | 0.396409 |
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Target: 5'- uGCGUGGCCGUCAUAaagaaaucgacGGCGGCGa--- -3' miRNA: 3'- uCGUGCCGGUAGUGU-----------UCGUCGCaaua -5' |
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| 1260 | 3' | -53.4 | NC_001317.1 | + | 16697 | 0.72 | 0.274489 |
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Target: 5'- uGCACGGCCAgcUCACcGGCcucGCGUUu- -3' miRNA: 3'- uCGUGCCGGU--AGUGuUCGu--CGCAAua -5' |
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| 1260 | 3' | -53.4 | NC_001317.1 | + | 8289 | 1.05 | 0.000945 |
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Target: 5'- gAGCACGGCCAUCACAAGCAGCGUUAUc -3' miRNA: 3'- -UCGUGCCGGUAGUGUUCGUCGCAAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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