Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12691 | 3' | -55.1 | NC_003347.1 | + | 7281 | 0.66 | 0.215994 |
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Target: 5'- gGGGGGCUGAgCGGUgGGGA---CAGCg -3' miRNA: 3'- -UCCCUGACUgGCUGgCUCUugaGUCG- -5' |
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| 12691 | 3' | -55.1 | NC_003347.1 | + | 6515 | 0.67 | 0.179348 |
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Target: 5'- cAGGGagcggagcGCUGACCaagcgagagucaGAgCGAGAGCUagagaCAGCa -3' miRNA: 3'- -UCCC--------UGACUGG------------CUgGCUCUUGA-----GUCG- -5' |
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| 12691 | 3' | -55.1 | NC_003347.1 | + | 805 | 0.67 | 0.179348 |
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Target: 5'- cGGGGAU--GCCGGCUugguugagggcgGAGGGC-CAGCg -3' miRNA: 3'- -UCCCUGacUGGCUGG------------CUCUUGaGUCG- -5' |
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| 12691 | 3' | -55.1 | NC_003347.1 | + | 6889 | 0.67 | 0.173805 |
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Target: 5'- uGGGGUUGA--GGCUgGAGAGCUCGGCg -3' miRNA: 3'- uCCCUGACUggCUGG-CUCUUGAGUCG- -5' |
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| 12691 | 3' | -55.1 | NC_003347.1 | + | 3414 | 0.68 | 0.153132 |
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Target: 5'- gGGGGGCgGGCgGGa-GGGAAgUCGGCg -3' miRNA: 3'- -UCCCUGaCUGgCUggCUCUUgAGUCG- -5' |
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| 12691 | 3' | -55.1 | NC_003347.1 | + | 3235 | 0.68 | 0.148324 |
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Target: 5'- gAGGGGCUGGCgGuugaGCCGGaAGCggagggCGGCg -3' miRNA: 3'- -UCCCUGACUGgC----UGGCUcUUGa-----GUCG- -5' |
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| 12691 | 3' | -55.1 | NC_003347.1 | + | 5297 | 0.68 | 0.137342 |
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Target: 5'- gGGGGuCUGGCCGGCguggauguaGAGAugggcggggcgguCUCGGCu -3' miRNA: 3'- -UCCCuGACUGGCUGg--------CUCUu------------GAGUCG- -5' |
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| 12691 | 3' | -55.1 | NC_003347.1 | + | 1209 | 0.71 | 0.088092 |
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Target: 5'- gGGGuGGCUG-CCGAUgGuGGAUUCGGCc -3' miRNA: 3'- -UCC-CUGACuGGCUGgCuCUUGAGUCG- -5' |
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| 12691 | 3' | -55.1 | NC_003347.1 | + | 5860 | 1.13 | 0.000031 |
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Target: 5'- gAGGGACUGACCGACCGAGAACUCAGCg -3' miRNA: 3'- -UCCCUGACUGGCUGGCUCUUGAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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