Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13016 | 3' | -56 | NC_003390.1 | + | 5523 | 0.66 | 0.637135 |
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Target: 5'- gGCagaUGAUCgAGGAuggcuCUGUGCCCCUc -3' miRNA: 3'- gUGag-ACUAGgUCCU-----GAUACGGGGGc -5' |
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| 13016 | 3' | -56 | NC_003390.1 | + | 18056 | 0.66 | 0.603851 |
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Target: 5'- gGCUUggaGAUCCAGGG-UGUaCCCCCa -3' miRNA: 3'- gUGAGa--CUAGGUCCUgAUAcGGGGGc -5' |
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| 13016 | 3' | -56 | NC_003390.1 | + | 2829 | 0.66 | 0.592793 |
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Target: 5'- -cCUUgcgGAUCaUGGGuGCUGUGCCCCUGa -3' miRNA: 3'- guGAGa--CUAG-GUCC-UGAUACGGGGGC- -5' |
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| 13016 | 3' | -56 | NC_003390.1 | + | 4525 | 0.67 | 0.548984 |
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Target: 5'- cCAUUCUGG-CCuGGuCUG-GCCCCCu -3' miRNA: 3'- -GUGAGACUaGGuCCuGAUaCGGGGGc -5' |
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| 13016 | 3' | -56 | NC_003390.1 | + | 34246 | 0.67 | 0.538179 |
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Target: 5'- aCACUCcgcagcGGUCCuAGGACg--GCCCUCa -3' miRNA: 3'- -GUGAGa-----CUAGG-UCCUGauaCGGGGGc -5' |
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| 13016 | 3' | -56 | NC_003390.1 | + | 7105 | 1.09 | 0.000682 |
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Target: 5'- cCACUCUGAUCCAGGACUAUGCCCCCGa -3' miRNA: 3'- -GUGAGACUAGGUCCUGAUACGGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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