Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13230 | 5' | -54.7 | NC_003401.1 | + | 94921 | 0.66 | 0.920656 |
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Target: 5'- cGAGGGUCagGCUucCGUGUGuAACuUGGCa -3' miRNA: 3'- aCUCCCAGg-CGA--GCGUAU-UUG-ACCGa -5' |
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| 13230 | 5' | -54.7 | NC_003401.1 | + | 91569 | 0.66 | 0.908665 |
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Target: 5'- gGAGGcGUCC-CUCGC-----CUGGCUa -3' miRNA: 3'- aCUCC-CAGGcGAGCGuauuuGACCGA- -5' |
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| 13230 | 5' | -54.7 | NC_003401.1 | + | 101604 | 0.67 | 0.887422 |
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Target: 5'- aUGAGGGcCaCGCaCGCAgaugcgcaaaaaAAACUGGCg -3' miRNA: 3'- -ACUCCCaG-GCGaGCGUa-----------UUUGACCGa -5' |
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| 13230 | 5' | -54.7 | NC_003401.1 | + | 18137 | 0.67 | 0.874397 |
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Target: 5'- gGAGcGG-CCGCUCGgGgcGAuCUGGCg -3' miRNA: 3'- aCUC-CCaGGCGAGCgUauUU-GACCGa -5' |
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| 13230 | 5' | -54.7 | NC_003401.1 | + | 94225 | 0.67 | 0.866838 |
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Target: 5'- cUGAGaGGUuccggCCGUUgGCAUcagcgccaaAAACUGGCUg -3' miRNA: 3'- -ACUC-CCA-----GGCGAgCGUA---------UUUGACCGA- -5' |
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| 13230 | 5' | -54.7 | NC_003401.1 | + | 121928 | 0.68 | 0.851059 |
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Target: 5'- cGGGGGg-CGCgggUGCGgcGGCUGGCg -3' miRNA: 3'- aCUCCCagGCGa--GCGUauUUGACCGa -5' |
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| 13230 | 5' | -54.7 | NC_003401.1 | + | 109529 | 0.71 | 0.69012 |
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Target: 5'- uUGGGGGauggccucgggCCGCUUGCcUAAcCUGGCUg -3' miRNA: 3'- -ACUCCCa----------GGCGAGCGuAUUuGACCGA- -5' |
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| 13230 | 5' | -54.7 | NC_003401.1 | + | 54009 | 0.72 | 0.637818 |
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Target: 5'- aGcGGGcCCGCUCGCGUuuGGACUGcGUUg -3' miRNA: 3'- aCuCCCaGGCGAGCGUA--UUUGAC-CGA- -5' |
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| 13230 | 5' | -54.7 | NC_003401.1 | + | 88875 | 1.07 | 0.003857 |
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Target: 5'- cUGAGGGUCCGCUCGCAUAAACUGGCUu -3' miRNA: 3'- -ACUCCCAGGCGAGCGUAUUUGACCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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