Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13545 | 3' | -54.5 | NC_003444.1 | + | 1553 | 0.66 | 0.623822 |
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Target: 5'- -uGCCaGACGGGCGUuauuuuucgCACGCugcugcUGACu -3' miRNA: 3'- guCGG-CUGCCCGUA---------GUGCGuau---ACUG- -5' |
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| 13545 | 3' | -54.5 | NC_003444.1 | + | 847 | 0.66 | 0.615796 |
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Target: 5'- cCAGCgUGAUGGGCAUCcucuugaAUGCccacucccccuugguGUGUGGCa -3' miRNA: 3'- -GUCG-GCUGCCCGUAG-------UGCG---------------UAUACUG- -5' |
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| 13545 | 3' | -54.5 | NC_003444.1 | + | 7256 | 0.67 | 0.565659 |
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Target: 5'- -uGCUGGCGGGUAUCGucuucggucagcuCGCugAUGACc -3' miRNA: 3'- guCGGCUGCCCGUAGU-------------GCGuaUACUG- -5' |
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| 13545 | 3' | -54.5 | NC_003444.1 | + | 37010 | 0.68 | 0.500311 |
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Target: 5'- aAGCCGuucACGGGcCGUCuuCGCcuUAUGGCa -3' miRNA: 3'- gUCGGC---UGCCC-GUAGu-GCGu-AUACUG- -5' |
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| 13545 | 3' | -54.5 | NC_003444.1 | + | 3421 | 0.7 | 0.37947 |
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Target: 5'- uUAGCCGcCGGGUAUUACGUcagguucaAUcUGACg -3' miRNA: 3'- -GUCGGCuGCCCGUAGUGCG--------UAuACUG- -5' |
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| 13545 | 3' | -54.5 | NC_003444.1 | + | 6438 | 1.1 | 0.000532 |
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Target: 5'- cCAGCCGACGGGCAUCACGCAUAUGACg -3' miRNA: 3'- -GUCGGCUGCCCGUAGUGCGUAUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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