Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1367 | 5' | -57.3 | NC_001335.1 | + | 7002 | 0.66 | 0.569154 |
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Target: 5'- aGC-CGGCGgaucacgcccucGCGGUagauguaCUGGcCCUGGUCa -3' miRNA: 3'- -CGaGUCGC------------UGCCA-------GACCaGGACCAGa -5' |
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| 1367 | 5' | -57.3 | NC_001335.1 | + | 7178 | 0.67 | 0.559519 |
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Target: 5'- --aCAGCGAgaaCGuguucuucGUCUGGUCCUGG-CUg -3' miRNA: 3'- cgaGUCGCU---GC--------CAGACCAGGACCaGA- -5' |
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| 1367 | 5' | -57.3 | NC_001335.1 | + | 12574 | 0.69 | 0.409114 |
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Target: 5'- cCUCAGgaGCGGUCuUGGUCUUGGUg- -3' miRNA: 3'- cGAGUCgcUGCCAG-ACCAGGACCAga -5' |
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| 1367 | 5' | -57.3 | NC_001335.1 | + | 24084 | 0.71 | 0.316155 |
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Target: 5'- uGCUCcuggcGGCGGCGGggCUGGUgcCCaGGUCUc -3' miRNA: 3'- -CGAG-----UCGCUGCCa-GACCA--GGaCCAGA- -5' |
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| 1367 | 5' | -57.3 | NC_001335.1 | + | 22553 | 1.1 | 0.000551 |
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Target: 5'- gGCUCAGCGACGGUCUGGUCCUGGUCUg -3' miRNA: 3'- -CGAGUCGCUGCCAGACCAGGACCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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