Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14663 | 3' | -51.9 | NC_003525.1 | + | 21486 | 1.1 | 0.002111 |
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Target: 5'- cUGAUUUUCAUCCGGCGUACGCGCCACu -3' miRNA: 3'- -ACUAAAAGUAGGCCGCAUGCGCGGUG- -5' |
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| 14663 | 3' | -51.9 | NC_003525.1 | + | 3799 | 0.74 | 0.480486 |
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Target: 5'- ------gCAUCCGGCGUcgGUGCCACa -3' miRNA: 3'- acuaaaaGUAGGCCGCAugCGCGGUG- -5' |
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| 14663 | 3' | -51.9 | NC_003525.1 | + | 24271 | 0.72 | 0.555495 |
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Target: 5'- cGGUaaaCAUCgGGCGUACGcCGCCGg -3' miRNA: 3'- aCUAaaaGUAGgCCGCAUGC-GCGGUg -5' |
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| 14663 | 3' | -51.9 | NC_003525.1 | + | 55716 | 0.7 | 0.711451 |
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Target: 5'- gUGAUUUa---CCGGCGUAC-CGCCAa -3' miRNA: 3'- -ACUAAAaguaGGCCGCAUGcGCGGUg -5' |
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| 14663 | 3' | -51.9 | NC_003525.1 | + | 27115 | 0.69 | 0.754444 |
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Target: 5'- aGAaUUUCAgcgauUCCGGUGUGauauccacCGCGCCGg -3' miRNA: 3'- aCUaAAAGU-----AGGCCGCAU--------GCGCGGUg -5' |
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| 14663 | 3' | -51.9 | NC_003525.1 | + | 41975 | 0.69 | 0.764899 |
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Target: 5'- cUGGUUUUCGUUUauacgaugaugGGCGgaaaagGCGUGCUACg -3' miRNA: 3'- -ACUAAAAGUAGG-----------CCGCa-----UGCGCGGUG- -5' |
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| 14663 | 3' | -51.9 | NC_003525.1 | + | 3135 | 0.67 | 0.842449 |
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Target: 5'- ---aUUUCAUCCGGUGauuUugGCGUCc- -3' miRNA: 3'- acuaAAAGUAGGCCGC---AugCGCGGug -5' |
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| 14663 | 3' | -51.9 | NC_003525.1 | + | 22530 | 0.67 | 0.868026 |
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Target: 5'- gGAUUUugUCAUCCGGUG-ACgGUGCUg- -3' miRNA: 3'- aCUAAA--AGUAGGCCGCaUG-CGCGGug -5' |
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| 14663 | 3' | -51.9 | NC_003525.1 | + | 38200 | 0.66 | 0.898535 |
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Target: 5'- gGAUaa-UGUCCGGUGUcAUGCuGCCACc -3' miRNA: 3'- aCUAaaaGUAGGCCGCA-UGCG-CGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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