miRNA: 3'- -AGUCUCCaCGCG---GGCAGuaGCGGaag -5'
miRNA: 3'- -AGUcucCACGCGGGCAGuAGCGGAAG- -5'
miRNA: 3'- -AGUCUCCACGCGGGCAGUAGCGGAAG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14692 | 3' | -57.3 | NC_003525.1 | + | 53946 | 0.67 | 0.588508 |
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Target: 5'- aUCAGGGG-GCGCguuCUGUUcgCGCCg-- -3' miRNA: 3'- -AGUCUCCaCGCG---GGCAGuaGCGGaag -5' |
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| 14692 | 3' | -57.3 | NC_003525.1 | + | 38592 | 0.76 | 0.174813 |
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Target: 5'- uUCA---GUGUGCCCGUC-UCGCCUUCg -3' miRNA: 3'- -AGUcucCACGCGGGCAGuAGCGGAAG- -5' |
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| 14692 | 3' | -57.3 | NC_003525.1 | + | 56101 | 1.09 | 0.000695 |
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Target: 5'- aUCAGAGGUGCGCCCGUCAUCGCCUUCc -3' miRNA: 3'- -AGUCUCCACGCGGGCAGUAGCGGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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