Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14704 | 3' | -49 | NC_003529.1 | + | 68893 | 0.66 | 0.999029 |
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Target: 5'- -uGCCuUUGccGACAcuuUCGAUGGGUu -3' miRNA: 3'- cuCGG-AACuaCUGUuuuAGCUGCCCG- -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 78365 | 0.66 | 0.998561 |
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Target: 5'- cGAGCCUU--UGACccguAUCGAuaUGGGUa -3' miRNA: 3'- -CUCGGAAcuACUGuuu-UAGCU--GCCCG- -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 145220 | 0.66 | 0.998561 |
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Target: 5'- cGGCCgcugcGGCAAAAcacUCGAUGGGUu -3' miRNA: 3'- cUCGGaacuaCUGUUUU---AGCUGCCCG- -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 113125 | 0.66 | 0.998262 |
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Target: 5'- aGAGCCauaUUGAUGACAcguuAUCGACa--- -3' miRNA: 3'- -CUCGG---AACUACUGUuu--UAGCUGcccg -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 131576 | 0.66 | 0.997914 |
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Target: 5'- -cGCCaagUGAUaGACAAGAaCGAaauuaacuCGGGCg -3' miRNA: 3'- cuCGGa--ACUA-CUGUUUUaGCU--------GCCCG- -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 48598 | 0.67 | 0.995175 |
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Target: 5'- -uGCCUcuccGGUGACAAAGUgGaACGuGGCc -3' miRNA: 3'- cuCGGAa---CUACUGUUUUAgC-UGC-CCG- -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 44976 | 0.68 | 0.99347 |
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Target: 5'- -uGCCcucGAUGAUGAuuUCGGUGGGCg -3' miRNA: 3'- cuCGGaa-CUACUGUUuuAGCUGCCCG- -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 128662 | 0.68 | 0.992453 |
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Target: 5'- uAGCUUUuuucAUGGCGAAAUgacggUGGCGGGCa -3' miRNA: 3'- cUCGGAAc---UACUGUUUUA-----GCUGCCCG- -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 20341 | 0.7 | 0.973986 |
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Target: 5'- aAGUacgUGGUGACcacGAGUUGGCGGGUg -3' miRNA: 3'- cUCGga-ACUACUGu--UUUAGCUGCCCG- -5' |
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| 14704 | 3' | -49 | NC_003529.1 | + | 65500 | 1.14 | 0.00663 |
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Target: 5'- cGAGCCUUGAUGACAAAAUCGACGGGCg -3' miRNA: 3'- -CUCGGAACUACUGUUUUAGCUGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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