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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14718 | 5' | -49.6 | NC_003529.1 | + | 143601 | 0.66 | 0.997316 |
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Target: 5'- cAACUGcUCGcgaccGUACACCGACAUGc- -3' miRNA: 3'- -UUGACcAGCuu---UAUGUGGCUGUGCaa -5' |
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| 14718 | 5' | -49.6 | NC_003529.1 | + | 20794 | 0.66 | 0.995574 |
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Target: 5'- --gUGGUCGAu-UACACCGAaGCGc- -3' miRNA: 3'- uugACCAGCUuuAUGUGGCUgUGCaa -5' |
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| 14718 | 5' | -49.6 | NC_003529.1 | + | 133445 | 0.68 | 0.984383 |
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Target: 5'- gAACUGGaagUCaAAGUACACCGAUACu-- -3' miRNA: 3'- -UUGACC---AGcUUUAUGUGGCUGUGcaa -5' |
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| 14718 | 5' | -49.6 | NC_003529.1 | + | 85568 | 0.68 | 0.980193 |
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Target: 5'- ---cGGUCGGAAUGCcgguGCCGGCGCc-- -3' miRNA: 3'- uugaCCAGCUUUAUG----UGGCUGUGcaa -5' |
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| 14718 | 5' | -49.6 | NC_003529.1 | + | 119217 | 0.69 | 0.977808 |
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Target: 5'- cAACUGGUCGAcg-ACugUgguaagGACACGUUg -3' miRNA: 3'- -UUGACCAGCUuuaUGugG------CUGUGCAA- -5' |
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| 14718 | 5' | -49.6 | NC_003529.1 | + | 47622 | 0.7 | 0.962639 |
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Target: 5'- cAAUUGGcacguacaugUUGAccGAUACACCGACACGa- -3' miRNA: 3'- -UUGACC----------AGCU--UUAUGUGGCUGUGCaa -5' |
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| 14718 | 5' | -49.6 | NC_003529.1 | + | 55013 | 1.03 | 0.026045 |
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Target: 5'- cAACUGGUCGAAAUACACCGACACGUUc -3' miRNA: 3'- -UUGACCAGCUUUAUGUGGCUGUGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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