Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1502 | 5' | -60.4 | NC_001335.1 | + | 29072 | 0.66 | 0.406452 |
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Target: 5'- -gGGaUCGAgaUCCCCaCUGCcucagcgAGAGCCUUc -3' miRNA: 3'- aaCC-AGCUg-AGGGG-GACG-------UCUCGGAG- -5' |
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| 1502 | 5' | -60.4 | NC_001335.1 | + | 34673 | 0.66 | 0.389279 |
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Target: 5'- -aGGUCGuaaaCCCCCUGCGG-GUCg- -3' miRNA: 3'- aaCCAGCuga-GGGGGACGUCuCGGag -5' |
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| 1502 | 5' | -60.4 | NC_001335.1 | + | 18032 | 0.67 | 0.354714 |
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Target: 5'- cUGGUCGAgCUggUUCUGCAGcGCCUCu -3' miRNA: 3'- aACCAGCU-GAggGGGACGUCuCGGAG- -5' |
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| 1502 | 5' | -60.4 | NC_001335.1 | + | 22412 | 0.67 | 0.346419 |
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Target: 5'- -cGGUCGaucaGCUCCCCCUcCGGGGUg-- -3' miRNA: 3'- aaCCAGC----UGAGGGGGAcGUCUCGgag -5' |
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| 1502 | 5' | -60.4 | NC_001335.1 | + | 50449 | 0.69 | 0.264434 |
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Target: 5'- -gGGuUCGACUCCCUCU-CGGGGCgCUUu -3' miRNA: 3'- aaCC-AGCUGAGGGGGAcGUCUCG-GAG- -5' |
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| 1502 | 5' | -60.4 | NC_001335.1 | + | 19433 | 0.71 | 0.169473 |
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Target: 5'- aUGG-CGACUUCCgCCUGCGcGGCCUg -3' miRNA: 3'- aACCaGCUGAGGG-GGACGUcUCGGAg -5' |
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| 1502 | 5' | -60.4 | NC_001335.1 | + | 35787 | 1.06 | 0.00035 |
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Target: 5'- aUUGGUCGACUCCCCCUGCAGAGCCUCu -3' miRNA: 3'- -AACCAGCUGAGGGGGACGUCUCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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