Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15641 | 5' | -45.5 | NC_004062.1 | + | 9339 | 1.15 | 0.010274 |
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Target: 5'- cGUUCGCAAACAAAACGAAACUGGGGCa -3' miRNA: 3'- -CAAGCGUUUGUUUUGCUUUGACCCCG- -5' |
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| 15641 | 5' | -45.5 | NC_004062.1 | + | 9440 | 0.84 | 0.533518 |
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Target: 5'- aUUUG-AGACAcAACGAAACUGGGGCg -3' miRNA: 3'- cAAGCgUUUGUuUUGCUUUGACCCCG- -5' |
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| 15641 | 5' | -45.5 | NC_004062.1 | + | 41686 | 0.69 | 0.998087 |
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Target: 5'- gGUUC-CGAGCGguucAGAUGAAACUgauGGGGUa -3' miRNA: 3'- -CAAGcGUUUGU----UUUGCUUUGA---CCCCG- -5' |
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| 15641 | 5' | -45.5 | NC_004062.1 | + | 4952 | 0.68 | 0.999151 |
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Target: 5'- --cCGcCAGACAAGACGAAACcGGuuaGCg -3' miRNA: 3'- caaGC-GUUUGUUUUGCUUUGaCCc--CG- -5' |
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| 15641 | 5' | -45.5 | NC_004062.1 | + | 104394 | 0.68 | 0.999456 |
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Target: 5'- -gUCGUugucacgauaaAAAUAAAACGuauaauuGGCUGGGGUu -3' miRNA: 3'- caAGCG-----------UUUGUUUUGCu------UUGACCCCG- -5' |
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| 15641 | 5' | -45.5 | NC_004062.1 | + | 107693 | 0.67 | 0.999735 |
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Target: 5'- --cCGCAGACGAAG-GAAucaUGGaGGCg -3' miRNA: 3'- caaGCGUUUGUUUUgCUUug-ACC-CCG- -5' |
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| 15641 | 5' | -45.5 | NC_004062.1 | + | 12447 | 0.66 | 0.999909 |
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Target: 5'- -----aAAACGAAuCGGGAUUGGGGUa -3' miRNA: 3'- caagcgUUUGUUUuGCUUUGACCCCG- -5' |
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| 15641 | 5' | -45.5 | NC_004062.1 | + | 17948 | 0.66 | 0.999963 |
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Target: 5'- -gUUGUAAugAAGACGAGucGCUGcaguGGGUu -3' miRNA: 3'- caAGCGUUugUUUUGCUU--UGAC----CCCG- -5' |
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| 15641 | 5' | -45.5 | NC_004062.1 | + | 3000 | 0.66 | 0.999963 |
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Target: 5'- -aUCGaCGAuaauuuacGCAAAAUGAucgacacaaugGugUGGGGCu -3' miRNA: 3'- caAGC-GUU--------UGUUUUGCU-----------UugACCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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