Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16251 | 5' | -52.7 | NC_004084.1 | + | 35774 | 0.66 | 0.85082 |
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Target: 5'- cGUagAGGuUCGAGCCGgaGGCGccgggaagGACGc -3' miRNA: 3'- -CAagUCCuAGCUCGGCaaCCGC--------UUGU- -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 48580 | 0.67 | 0.814447 |
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Target: 5'- -aUCAGGA-CGcAGCCGacaccuaucgGGCGAACc -3' miRNA: 3'- caAGUCCUaGC-UCGGCaa--------CCGCUUGu -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 43601 | 0.67 | 0.813494 |
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Target: 5'- ---uGGGAUCGcgaguacGGCCGcUGGcCGGACAa -3' miRNA: 3'- caagUCCUAGC-------UCGGCaACC-GCUUGU- -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 8468 | 0.67 | 0.804832 |
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Target: 5'- --aUAGGAUCgGGGCUG-UGGcCGGACGa -3' miRNA: 3'- caaGUCCUAG-CUCGGCaACC-GCUUGU- -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 49636 | 0.67 | 0.79503 |
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Target: 5'- -aUguGGAccugcUCGAGCUGUgGGCGGugGu -3' miRNA: 3'- caAguCCU-----AGCUCGGCAaCCGCUugU- -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 4580 | 0.68 | 0.732927 |
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Target: 5'- aGUUC-GGAuUCGAGCCGUUcccGGagGAACAc -3' miRNA: 3'- -CAAGuCCU-AGCUCGGCAA---CCg-CUUGU- -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 40435 | 0.68 | 0.722139 |
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Target: 5'- uGUUCGaGAUCGAgGCCGagGGCG-ACAc -3' miRNA: 3'- -CAAGUcCUAGCU-CGGCaaCCGCuUGU- -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 22819 | 0.7 | 0.633499 |
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Target: 5'- -gUCAcGGAucgauccguUCGAGCCGccgaGGCGAACGu -3' miRNA: 3'- caAGU-CCU---------AGCUCGGCaa--CCGCUUGU- -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 35214 | 0.72 | 0.523007 |
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Target: 5'- -gUCGGGAUCGAGCCGacgaucGCGAuCGa -3' miRNA: 3'- caAGUCCUAGCUCGGCaac---CGCUuGU- -5' |
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| 16251 | 5' | -52.7 | NC_004084.1 | + | 57225 | 1.09 | 0.00195 |
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Target: 5'- cGUUCAGGAUCGAGCCGUUGGCGAACAc -3' miRNA: 3'- -CAAGUCCUAGCUCGGCAACCGCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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