Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16263 | 5' | -57.5 | NC_004084.1 | + | 39361 | 0.73 | 0.25245 |
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Target: 5'- uGCCCgUUCGUGaGGACg-ACGCCGUCu -3' miRNA: 3'- -CGGGaAGGCGC-CCUGaaUGCGGCAGu -5' |
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| 16263 | 5' | -57.5 | NC_004084.1 | + | 28973 | 0.68 | 0.492429 |
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Target: 5'- cGCCCacgccgcgUUCGCGGuGAagcuCGCCGUCGa -3' miRNA: 3'- -CGGGa-------AGGCGCC-CUgaauGCGGCAGU- -5' |
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| 16263 | 5' | -57.5 | NC_004084.1 | + | 47982 | 0.67 | 0.585948 |
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Target: 5'- --aCUUCUGCGGGAUgcACGaggaCGUCGa -3' miRNA: 3'- cggGAAGGCGCCCUGaaUGCg---GCAGU- -5' |
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| 16263 | 5' | -57.5 | NC_004084.1 | + | 39846 | 0.66 | 0.618035 |
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Target: 5'- aGUCCaUCgGCGGccgcgGCGCCGUCGa -3' miRNA: 3'- -CGGGaAGgCGCCcugaaUGCGGCAGU- -5' |
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| 16263 | 5' | -57.5 | NC_004084.1 | + | 58276 | 0.66 | 0.649154 |
|
Target: 5'- uGCCCUggCUGCGGGcGCUcUGCGgacgaggucuccuCCGUCc -3' miRNA: 3'- -CGGGAa-GGCGCCC-UGA-AUGC-------------GGCAGu -5' |
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| 16263 | 5' | -57.5 | NC_004084.1 | + | 15382 | 0.71 | 0.346338 |
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Target: 5'- cGCuCCUUCaCGCGGGuccGCUUGCGUC-UCGu -3' miRNA: 3'- -CG-GGAAG-GCGCCC---UGAAUGCGGcAGU- -5' |
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| 16263 | 5' | -57.5 | NC_004084.1 | + | 51664 | 1.1 | 0.000635 |
|
Target: 5'- uGCCCUUCCGCGGGACUUACGCCGUCAu -3' miRNA: 3'- -CGGGAAGGCGCCCUGAAUGCGGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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