Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16319 | 5' | -60 | NC_004084.1 | + | 14773 | 0.66 | 0.492088 |
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Target: 5'- cCGCCCGUuCGUGCCUau-CGAGaCGc -3' miRNA: 3'- -GCGGGCGuGCACGGAaguGCUCcGCa -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 26266 | 0.66 | 0.491095 |
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Target: 5'- uCGCCCGUgucccagAUgGUGCCgcUCGCGAGGUc- -3' miRNA: 3'- -GCGGGCG-------UG-CACGGa-AGUGCUCCGca -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 11732 | 0.66 | 0.472421 |
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Target: 5'- uCGCCgGCgACGUGCUcgaaUCGCGGacuguGGCGg -3' miRNA: 3'- -GCGGgCG-UGCACGGa---AGUGCU-----CCGCa -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 57749 | 0.66 | 0.472421 |
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Target: 5'- gGCCUGCuCGUgGCgUUCACGgaggagcuugcGGGCGa -3' miRNA: 3'- gCGGGCGuGCA-CGgAAGUGC-----------UCCGCa -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 33800 | 0.66 | 0.462737 |
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Target: 5'- uCGCCCGguuCGUGCCggagUCAUccGGUGUg -3' miRNA: 3'- -GCGGGCgu-GCACGGa---AGUGcuCCGCA- -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 44244 | 0.67 | 0.453158 |
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Target: 5'- aGCUCGUcuacgacgggACGaccgGCCUUCACGAcgacGGCGa -3' miRNA: 3'- gCGGGCG----------UGCa---CGGAAGUGCU----CCGCa -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 30453 | 0.67 | 0.434332 |
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Target: 5'- aCGuCCCGCuggggACGUuCCUcUACGAGGCGc -3' miRNA: 3'- -GC-GGGCG-----UGCAcGGAaGUGCUCCGCa -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 210 | 0.71 | 0.24802 |
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Target: 5'- aGCCCGCACG-GCCaUCgGCGGGGgaGUc -3' miRNA: 3'- gCGGGCGUGCaCGGaAG-UGCUCCg-CA- -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 53898 | 0.74 | 0.147904 |
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Target: 5'- uCGCCUGCACGcGCU---GCGAGGCGa -3' miRNA: 3'- -GCGGGCGUGCaCGGaagUGCUCCGCa -5' |
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| 16319 | 5' | -60 | NC_004084.1 | + | 24322 | 1.06 | 0.000628 |
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Target: 5'- uCGCCCGCACGUGCCUUCACGAGGCGUc -3' miRNA: 3'- -GCGGGCGUGCACGGAAGUGCUCCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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