Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16373 | 3' | -65.4 | NC_004084.1 | + | 39738 | 0.66 | 0.274758 |
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Target: 5'- cGGGGuuGGAGCGCGCgaguuccuCGGCguCgCGUa -3' miRNA: 3'- -CCCCcuCCUCGCGCG--------GCCGguG-GCAa -5' |
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| 16373 | 3' | -65.4 | NC_004084.1 | + | 16281 | 0.67 | 0.220978 |
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Target: 5'- -aGGGGGGAGUGCGCuCaGCgGCUGg- -3' miRNA: 3'- ccCCCUCCUCGCGCG-GcCGgUGGCaa -5' |
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| 16373 | 3' | -65.4 | NC_004084.1 | + | 27723 | 0.69 | 0.159327 |
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Target: 5'- cGGGuGAGGAGCcaggcgagaCGCUGGUCACCGc- -3' miRNA: 3'- cCCC-CUCCUCGc--------GCGGCCGGUGGCaa -5' |
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| 16373 | 3' | -65.4 | NC_004084.1 | + | 58488 | 0.7 | 0.126203 |
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Target: 5'- cGGGGGucuucgaagucgAGGu-CGUGUCGGCCACCGa- -3' miRNA: 3'- -CCCCC------------UCCucGCGCGGCCGGUGGCaa -5' |
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| 16373 | 3' | -65.4 | NC_004084.1 | + | 27628 | 0.71 | 0.122944 |
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Target: 5'- -cGGGAGGcccuuGUGCG-CGGCCACCGg- -3' miRNA: 3'- ccCCCUCCu----CGCGCgGCCGGUGGCaa -5' |
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| 16373 | 3' | -65.4 | NC_004084.1 | + | 3346 | 1.06 | 0.000199 |
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Target: 5'- cGGGGGAGGAGCGCGCCGGCCACCGUUc -3' miRNA: 3'- -CCCCCUCCUCGCGCGGCCGGUGGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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