Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16428 | 3' | -56.2 | NC_004084.1 | + | 20673 | 0.66 | 0.637895 |
Target: 5'- -cGAUCGCGCGCgUACGa-GCuaCUCCAGg -3' miRNA: 3'- caUUAGCGUGCG-AUGCgaCG--GGGGUC- -5' |
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16428 | 3' | -56.2 | NC_004084.1 | + | 34571 | 0.69 | 0.467145 |
Target: 5'- -cGAgggCGCugGCgGCGaCUGCCUCCAu -3' miRNA: 3'- caUUa--GCGugCGaUGC-GACGGGGGUc -5' |
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16428 | 3' | -56.2 | NC_004084.1 | + | 56940 | 0.71 | 0.3643 |
Target: 5'- cGUAGUCGUcgucgGCGCUGaacUGCUGgUCCCAGu -3' miRNA: 3'- -CAUUAGCG-----UGCGAU---GCGACgGGGGUC- -5' |
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16428 | 3' | -56.2 | NC_004084.1 | + | 27703 | 0.71 | 0.347357 |
Target: 5'- ----gCGuCACGCgACGCUGUUCCCGGg -3' miRNA: 3'- cauuaGC-GUGCGaUGCGACGGGGGUC- -5' |
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16428 | 3' | -56.2 | NC_004084.1 | + | 34018 | 1.09 | 0.000823 |
Target: 5'- gGUAAUCGCACGCUACGCUGCCCCCAGa -3' miRNA: 3'- -CAUUAGCGUGCGAUGCGACGGGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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