miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16520 3' -57.1 NC_004102.1 + 1771 0.66 0.179379
Target:  5'- aAGCGGCCUCgacgaaCGCCCCUacugcUGGcacuacccuccaAGACCu -3'
miRNA:   3'- gUCGUUGGAG------GUGGGGA-----ACC------------UCUGGu -5'
16520 3' -57.1 NC_004102.1 + 3255 0.66 0.168637
Target:  5'- cCAGUcgUCUUC-UCCCgaaUGGAGACCAa -3'
miRNA:   3'- -GUCGuuGGAGGuGGGGa--ACCUCUGGU- -5'
16520 3' -57.1 NC_004102.1 + 6086 0.67 0.144266
Target:  5'- cCGGCuaauAGCCUUCGCCUCccgGGGGaACCAu -3'
miRNA:   3'- -GUCG----UUGGAGGUGGGGaa-CCUC-UGGU- -5'
16520 3' -57.1 NC_004102.1 + 8624 0.69 0.098387
Target:  5'- cCAGguA-CUCCGCCCCccccGGGGACCc -3'
miRNA:   3'- -GUCguUgGAGGUGGGGaa--CCUCUGGu -5'
16520 3' -57.1 NC_004102.1 + 106 0.75 0.033136
Target:  5'- -uGCAGCCUCCAggacccCCCCUcccgGGAGAgCCAu -3'
miRNA:   3'- guCGUUGGAGGU------GGGGAa---CCUCU-GGU- -5'
16520 3' -57.1 NC_004102.1 + 3426 1.08 5.2e-05
Target:  5'- cCAGCAACCUCCACCCCUUGGAGACCAu -3'
miRNA:   3'- -GUCGUUGGAGGUGGGGAACCUCUGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.