Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16530 | 3' | -56.6 | NC_004102.1 | + | 3243 | 0.66 | 0.192909 |
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Target: 5'- -cUCGCaGGCcguuGUGCGCCCAGUcucgaagaggggugAGAUGGu -3' miRNA: 3'- guAGUG-CCG----CAUGCGGGUCG--------------UCUGCU- -5' |
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| 16530 | 3' | -56.6 | NC_004102.1 | + | 224 | 0.66 | 0.188854 |
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Target: 5'- gAUUugGGCGUGC-CCCcGCAaGACu- -3' miRNA: 3'- gUAGugCCGCAUGcGGGuCGU-CUGcu -5' |
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| 16530 | 3' | -56.6 | NC_004102.1 | + | 7542 | 0.66 | 0.18319 |
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Target: 5'- gGUCAUGGuCG-ACGgUCAGUAGugGGg -3' miRNA: 3'- gUAGUGCC-GCaUGCgGGUCGUCugCU- -5' |
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| 16530 | 3' | -56.6 | NC_004102.1 | + | 3883 | 0.67 | 0.151801 |
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Target: 5'- --cCGCGGUGUGCaCCCguggaguggcuaaGGCGGugGAc -3' miRNA: 3'- guaGUGCCGCAUGcGGG-------------UCGUCugCU- -5' |
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| 16530 | 3' | -56.6 | NC_004102.1 | + | 3450 | 0.69 | 0.111133 |
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Target: 5'- --gCugGGCGUACGCCguGaUGGGCGc -3' miRNA: 3'- guaGugCCGCAUGCGGguC-GUCUGCu -5' |
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| 16530 | 3' | -56.6 | NC_004102.1 | + | 3421 | 1.09 | 6.1e-05 |
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Target: 5'- cCAUCACGGCGUACGCCCAGCAGACGAg -3' miRNA: 3'- -GUAGUGCCGCAUGCGGGUCGUCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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