miRNA: 3'- aAAAGGUCgCGAcuuuag-CCUUCAAC- -5'
miRNA: 3'- aaaAGGUCGCGACUUUAGc--CUUCaac -5'
miRNA: 3'- aAAAGGUCGCGACuuua-GCC-UUCAac -5'
miRNA: 3'- aAAAGGUCGCGaCUU----UAGCC--UUCAAc -5'
miRNA: 3'- -AAAAGGUCGCGACUUUAGCCUUCAAC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17747 | 5' | -47.5 | NC_004629.1 | + | 174281 | 0.67 | 0.999879 |
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Target: 5'- aUUUCCGGgGCUuccaaaaGGAAGUUGg -3' miRNA: 3'- aAAAGGUCgCGAcuuuag-CCUUCAAC- -5' |
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| 17747 | 5' | -47.5 | NC_004629.1 | + | 90638 | 0.69 | 0.999513 |
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Target: 5'- --aUCUAGUGCUGAAGUCaauGAAGg-- -3' miRNA: 3'- aaaAGGUCGCGACUUUAGc--CUUCaac -5' |
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| 17747 | 5' | -47.5 | NC_004629.1 | + | 240797 | 0.69 | 0.999083 |
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Target: 5'- aUUUCCAGCGUUGuguagCGGcAGGUg- -3' miRNA: 3'- aAAAGGUCGCGACuuua-GCC-UUCAac -5' |
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| 17747 | 5' | -47.5 | NC_004629.1 | + | 190479 | 0.7 | 0.997207 |
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Target: 5'- gUUUCCAGCGC-GAAcagcAUCGGcaAAGUUa -3' miRNA: 3'- aAAAGGUCGCGaCUU----UAGCC--UUCAAc -5' |
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| 17747 | 5' | -47.5 | NC_004629.1 | + | 10637 | 1.06 | 0.043497 |
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Target: 5'- gUUUUCCAGCGCUGAAAUCGGAAGUUGc -3' miRNA: 3'- -AAAAGGUCGCGACUUUAGCCUUCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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