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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17757 | 3' | -52.6 | NC_004629.1 | + | 96401 | 0.66 | 0.995166 |
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Target: 5'- gAGUAGCAGCUGGagucucaACaauAACGCCAu- -3' miRNA: 3'- -UCAUCGUUGACCga-----UGc--UUGCGGUca -5' |
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| 17757 | 3' | -52.6 | NC_004629.1 | + | 274649 | 0.66 | 0.992605 |
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Target: 5'- ---uGC-ACUGGacgAUGAACGCCAGa -3' miRNA: 3'- ucauCGuUGACCga-UGCUUGCGGUCa -5' |
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| 17757 | 3' | -52.6 | NC_004629.1 | + | 198665 | 0.68 | 0.980392 |
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Target: 5'- uGUGGUAACUGGUUAU-AAUGCuCAGa -3' miRNA: 3'- uCAUCGUUGACCGAUGcUUGCG-GUCa -5' |
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| 17757 | 3' | -52.6 | NC_004629.1 | + | 269261 | 0.69 | 0.967399 |
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Target: 5'- aAGUAGcCAGCUGGCUACuuuuaGAAauugGCUGGUa -3' miRNA: 3'- -UCAUC-GUUGACCGAUG-----CUUg---CGGUCA- -5' |
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| 17757 | 3' | -52.6 | NC_004629.1 | + | 259084 | 0.69 | 0.967399 |
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Target: 5'- uGUAGauaGACUGGCgguauuugGCGAGCGCUcuaaAGUu -3' miRNA: 3'- uCAUCg--UUGACCGa-------UGCUUGCGG----UCA- -5' |
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| 17757 | 3' | -52.6 | NC_004629.1 | + | 146747 | 1.08 | 0.011335 |
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Target: 5'- uAGUAGCAACUGGCUACGAACGCCAGUa -3' miRNA: 3'- -UCAUCGUUGACCGAUGCUUGCGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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