Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18050 | 3' | -53.3 | NC_004680.1 | + | 16849 | 0.66 | 0.852319 |
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Target: 5'- -uGGCGUGUaCcgGUUGAACcucgcCCUGCa -3' miRNA: 3'- guCCGCACA-GuaUAGCUUGc----GGACGc -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 6039 | 0.66 | 0.825732 |
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Target: 5'- gCGGGCGcagGUCGUcaaUGGGCGCgUGUGg -3' miRNA: 3'- -GUCCGCa--CAGUAua-GCUUGCGgACGC- -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 28610 | 0.66 | 0.816443 |
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Target: 5'- aAGGCGUcUCA--UCGAACGgCaUGCGu -3' miRNA: 3'- gUCCGCAcAGUauAGCUUGCgG-ACGC- -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 56860 | 0.67 | 0.797284 |
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Target: 5'- aGGGUGgagGUCAUGUCu--CGCCcgagGCGg -3' miRNA: 3'- gUCCGCa--CAGUAUAGcuuGCGGa---CGC- -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 56032 | 0.67 | 0.767258 |
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Target: 5'- -cGGCGUaGuUCGaGUCGGGCGCgUGCu -3' miRNA: 3'- guCCGCA-C-AGUaUAGCUUGCGgACGc -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 40853 | 0.67 | 0.756951 |
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Target: 5'- -cGGCG-GUCAUGUCG-AUGaCUGCa -3' miRNA: 3'- guCCGCaCAGUAUAGCuUGCgGACGc -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 5522 | 0.7 | 0.604309 |
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Target: 5'- gGGGCGUGUCGUugucgUGAACGCggagaugGCGu -3' miRNA: 3'- gUCCGCACAGUAua---GCUUGCGga-----CGC- -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 48102 | 0.71 | 0.57125 |
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Target: 5'- uGGGCGgaUGUCA---UGAGCGCCgGCGa -3' miRNA: 3'- gUCCGC--ACAGUauaGCUUGCGGaCGC- -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 45250 | 0.71 | 0.538645 |
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Target: 5'- uGGGCGgaGUCcUAUCGGGCGCaaGCGu -3' miRNA: 3'- gUCCGCa-CAGuAUAGCUUGCGgaCGC- -5' |
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| 18050 | 3' | -53.3 | NC_004680.1 | + | 1636 | 1.09 | 0.00164 |
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Target: 5'- cCAGGCGUGUCAUAUCGAACGCCUGCGa -3' miRNA: 3'- -GUCCGCACAGUAUAGCUUGCGGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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