Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18057 | 3' | -59.1 | NC_004680.1 | + | 53875 | 0.66 | 0.501175 |
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Target: 5'- cGGGCUgCACCU-GCCUcgGUGCCG-CAc -3' miRNA: 3'- cUCCGAgGUGGAgCGGG--UACGGUaGU- -5' |
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| 18057 | 3' | -59.1 | NC_004680.1 | + | 3811 | 0.67 | 0.451367 |
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Target: 5'- cGAGGuCUCCAagaUUCGCgguGUGCCGUCGg -3' miRNA: 3'- -CUCC-GAGGUg--GAGCGgg-UACGGUAGU- -5' |
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| 18057 | 3' | -59.1 | NC_004680.1 | + | 12656 | 0.67 | 0.441732 |
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Target: 5'- cGAGcGCaCCGCCgcCGUCCAUGCCgAUCc -3' miRNA: 3'- -CUC-CGaGGUGGa-GCGGGUACGG-UAGu -5' |
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| 18057 | 3' | -59.1 | NC_004680.1 | + | 42678 | 0.67 | 0.432213 |
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Target: 5'- uGGGCUUCGCCgcgGCUgAUGCCGUg- -3' miRNA: 3'- cUCCGAGGUGGag-CGGgUACGGUAgu -5' |
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| 18057 | 3' | -59.1 | NC_004680.1 | + | 25054 | 0.67 | 0.422816 |
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Target: 5'- -cGGCUCCACCgcUUGCUCcgGUCAcaUCGc -3' miRNA: 3'- cuCCGAGGUGG--AGCGGGuaCGGU--AGU- -5' |
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| 18057 | 3' | -59.1 | NC_004680.1 | + | 26894 | 0.68 | 0.395376 |
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Target: 5'- cGGGGCaCCACCacuugcggucaUCGCCgAUGCCGg-- -3' miRNA: 3'- -CUCCGaGGUGG-----------AGCGGgUACGGUagu -5' |
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| 18057 | 3' | -59.1 | NC_004680.1 | + | 11164 | 0.73 | 0.187862 |
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Target: 5'- cGGGGCcgCCACCaaggcCGCCCGgcagcaggaUGCCAUCGa -3' miRNA: 3'- -CUCCGa-GGUGGa----GCGGGU---------ACGGUAGU- -5' |
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| 18057 | 3' | -59.1 | NC_004680.1 | + | 2983 | 1.08 | 0.00047 |
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Target: 5'- uGAGGCUCCACCUCGCCCAUGCCAUCAg -3' miRNA: 3'- -CUCCGAGGUGGAGCGGGUACGGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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