Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18529 | 3' | -54 | NC_004681.1 | + | 35790 | 0.66 | 0.832585 |
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Target: 5'- uUCgUCCGA---CUCcauUGCGGGGCGCg- -3' miRNA: 3'- -AGgAGGCUaauGAG---ACGUCUCGCGag -5' |
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| 18529 | 3' | -54 | NC_004681.1 | + | 42461 | 0.67 | 0.8049 |
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Target: 5'- gCCUCCGAUgcgcggcACUUgcgGCugguggcauuGGAGCGCUg -3' miRNA: 3'- aGGAGGCUAa------UGAGa--CG----------UCUCGCGAg -5' |
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| 18529 | 3' | -54 | NC_004681.1 | + | 70696 | 0.67 | 0.775597 |
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Target: 5'- gUCCUCagccaugcgcUGAgcGCUCUGCAGAGUaaUCg -3' miRNA: 3'- -AGGAG----------GCUaaUGAGACGUCUCGcgAG- -5' |
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| 18529 | 3' | -54 | NC_004681.1 | + | 66894 | 0.68 | 0.744966 |
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Target: 5'- gCCUUgGcgUACUCgggGUccagGGAGUGCUCa -3' miRNA: 3'- aGGAGgCuaAUGAGa--CG----UCUCGCGAG- -5' |
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| 18529 | 3' | -54 | NC_004681.1 | + | 68453 | 0.68 | 0.744966 |
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Target: 5'- aUUgUCCGG--GCUCcGCAGAGCuCUCg -3' miRNA: 3'- -AGgAGGCUaaUGAGaCGUCUCGcGAG- -5' |
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| 18529 | 3' | -54 | NC_004681.1 | + | 37650 | 0.68 | 0.702588 |
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Target: 5'- -gCUCCGAc--UUCUGCGGGGC-CUCg -3' miRNA: 3'- agGAGGCUaauGAGACGUCUCGcGAG- -5' |
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| 18529 | 3' | -54 | NC_004681.1 | + | 2893 | 0.71 | 0.56078 |
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Target: 5'- gCgUCCGGUagaGCUCUGCGGugucgaugacGCGCUCa -3' miRNA: 3'- aGgAGGCUAa--UGAGACGUCu---------CGCGAG- -5' |
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| 18529 | 3' | -54 | NC_004681.1 | + | 49064 | 0.77 | 0.257935 |
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Target: 5'- aCCUCCGGUUGaUCuUGCgGGAGCGCUUg -3' miRNA: 3'- aGGAGGCUAAUgAG-ACG-UCUCGCGAG- -5' |
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| 18529 | 3' | -54 | NC_004681.1 | + | 70578 | 1.11 | 0.00123 |
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Target: 5'- aUCCUCCGAUUACUCUGCAGAGCGCUCa -3' miRNA: 3'- -AGGAGGCUAAUGAGACGUCUCGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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