Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18575 | 5' | -54.8 | NC_004682.1 | + | 46854 | 0.66 | 0.743492 |
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Target: 5'- gCUCGCAuGGCUGAUCUGGGaGGaCUCGa -3' miRNA: 3'- -GGGUGU-CUGACUGGGCUCaUC-GAGUg -5' |
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| 18575 | 5' | -54.8 | NC_004682.1 | + | 32126 | 0.66 | 0.732911 |
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Target: 5'- cUCCGC-GAUUucGGCCUGGGUgaagccAGCUCGCu -3' miRNA: 3'- -GGGUGuCUGA--CUGGGCUCA------UCGAGUG- -5' |
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| 18575 | 5' | -54.8 | NC_004682.1 | + | 18381 | 0.66 | 0.722228 |
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Target: 5'- aCCAUcguccuucgGGAC-GGCCCGA-UGGUUCACg -3' miRNA: 3'- gGGUG---------UCUGaCUGGGCUcAUCGAGUG- -5' |
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| 18575 | 5' | -54.8 | NC_004682.1 | + | 25274 | 0.66 | 0.700599 |
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Target: 5'- gCCCACGGcgUGGCCC-AGUGGCgcgugaGCg -3' miRNA: 3'- -GGGUGUCugACUGGGcUCAUCGag----UG- -5' |
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| 18575 | 5' | -54.8 | NC_004682.1 | + | 32081 | 0.66 | 0.687487 |
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Target: 5'- gCCC-CGGAC-GAUCCGAGacacgguugcggGGUUCACg -3' miRNA: 3'- -GGGuGUCUGaCUGGGCUCa-----------UCGAGUG- -5' |
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| 18575 | 5' | -54.8 | NC_004682.1 | + | 20419 | 0.67 | 0.667678 |
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Target: 5'- uCCCACGGGCauggacuaGGCCCuGGgGGCUCGa -3' miRNA: 3'- -GGGUGUCUGa-------CUGGGcUCaUCGAGUg -5' |
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| 18575 | 5' | -54.8 | NC_004682.1 | + | 21616 | 0.74 | 0.295458 |
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Target: 5'- uCUCGCAGAcCUGACCCGca-GGCUCAa -3' miRNA: 3'- -GGGUGUCU-GACUGGGCucaUCGAGUg -5' |
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| 18575 | 5' | -54.8 | NC_004682.1 | + | 33535 | 0.78 | 0.165042 |
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Target: 5'- gCCCGCGuuCUgGACCCGAGUgaacAGCUCGCc -3' miRNA: 3'- -GGGUGUcuGA-CUGGGCUCA----UCGAGUG- -5' |
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| 18575 | 5' | -54.8 | NC_004682.1 | + | 32476 | 1.1 | 0.000816 |
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Target: 5'- gCCCACAGACUGACCCGAGUAGCUCACc -3' miRNA: 3'- -GGGUGUCUGACUGGGCUCAUCGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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