Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18578 | 5' | -60.1 | NC_004682.1 | + | 50565 | 0.66 | 0.416613 |
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Target: 5'- uGGGuACUGgGGgUaCCGGGUagGCAGGGc -3' miRNA: 3'- -CCCuUGACgUCgA-GGCCCG--CGUCCUu -5' |
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| 18578 | 5' | -60.1 | NC_004682.1 | + | 38352 | 0.66 | 0.406465 |
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Target: 5'- uGGcGAACUGCAGCcCCGGcucguccccguccGCG-AGGAu -3' miRNA: 3'- -CC-CUUGACGUCGaGGCC-------------CGCgUCCUu -5' |
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| 18578 | 5' | -60.1 | NC_004682.1 | + | 45621 | 0.67 | 0.363199 |
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Target: 5'- aGGGACUGU--CUCCGGGUGUAGu-- -3' miRNA: 3'- cCCUUGACGucGAGGCCCGCGUCcuu -5' |
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| 18578 | 5' | -60.1 | NC_004682.1 | + | 31237 | 0.68 | 0.285217 |
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Target: 5'- cGGGAucagcuuCUGCAGCagaCGGGCuGCgAGGAu -3' miRNA: 3'- -CCCUu------GACGUCGag-GCCCG-CG-UCCUu -5' |
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| 18578 | 5' | -60.1 | NC_004682.1 | + | 46627 | 0.69 | 0.264551 |
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Target: 5'- aGGcGCaGCAuCUgCGGGCGCAGGAu -3' miRNA: 3'- cCCuUGaCGUcGAgGCCCGCGUCCUu -5' |
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| 18578 | 5' | -60.1 | NC_004682.1 | + | 5280 | 0.7 | 0.238906 |
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Target: 5'- uGGGGCUGCuGCUCaCGccggccccGGCGCAGGc- -3' miRNA: 3'- cCCUUGACGuCGAG-GC--------CCGCGUCCuu -5' |
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| 18578 | 5' | -60.1 | NC_004682.1 | + | 50459 | 0.72 | 0.174242 |
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Target: 5'- aGGGGA--GC-GCUCCGGGCGCucccGGAGg -3' miRNA: 3'- -CCCUUgaCGuCGAGGCCCGCGu---CCUU- -5' |
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| 18578 | 5' | -60.1 | NC_004682.1 | + | 40444 | 0.74 | 0.115621 |
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Target: 5'- uGGGGuc--CAGCUCCGGGUGguGGAGu -3' miRNA: 3'- -CCCUugacGUCGAGGCCCGCguCCUU- -5' |
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| 18578 | 5' | -60.1 | NC_004682.1 | + | 29959 | 1.07 | 0.000352 |
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Target: 5'- gGGGAACUGCAGCUCCGGGCGCAGGAAc -3' miRNA: 3'- -CCCUUGACGUCGAGGCCCGCGUCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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