Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19597 | 5' | -55.8 | NC_004686.1 | + | 21372 | 0.66 | 0.705504 |
Target: 5'- gCGGCUUAugGgUCGUCGGaguugggGGCGGUGUc -3' miRNA: 3'- -GCCGGGU--UgAGCAGUCa------CUGCCACAu -5' |
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19597 | 5' | -55.8 | NC_004686.1 | + | 22105 | 0.67 | 0.607246 |
Target: 5'- gGGCaCCAACUCGUCgagcAGUGGCGc---- -3' miRNA: 3'- gCCG-GGUUGAGCAG----UCACUGCcacau -5' |
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19597 | 5' | -55.8 | NC_004686.1 | + | 2073 | 0.75 | 0.221724 |
Target: 5'- uCGGUCCGGCUaucuccaUGUCGGgGGCGGUGUAu -3' miRNA: 3'- -GCCGGGUUGA-------GCAGUCaCUGCCACAU- -5' |
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19597 | 5' | -55.8 | NC_004686.1 | + | 24658 | 1.07 | 0.00124 |
Target: 5'- gCGGCCCAACUCGUCAGUGACGGUGUAg -3' miRNA: 3'- -GCCGGGUUGAGCAGUCACUGCCACAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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