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miRNA: 3'- gGCGgc--GG-UGGCGCU-GCGGCGu-------GUUG- -5'
miRNA: 3'- gGCGG---CGGUGG-------CGCUGCGG--CGUGUUG- -5'
miRNA: 3'- ggcGGCGGUGGCGCUGCGG------------CGUGUUG- -5'
miRNA: 3'- -GGCGGCGGUGGCGCUGCGGCGUGUUG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 20958 | 3' | -61.3 | NC_004724.1 | + | 11669 | 0.66 | 0.178642 |
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Target: 5'- gCGCacaaCCgACCGCGAcCGCCGCucggggauCAGCg -3' miRNA: 3'- gGCGgc--GG-UGGCGCU-GCGGCGu-------GUUG- -5' |
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| 20958 | 3' | -61.3 | NC_004724.1 | + | 8548 | 0.67 | 0.15452 |
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Target: 5'- aCGUCcuaGCCGCUcggagagGCGACGCCacGUGCGACg -3' miRNA: 3'- gGCGG---CGGUGG-------CGCUGCGG--CGUGUUG- -5' |
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| 20958 | 3' | -61.3 | NC_004724.1 | + | 3845 | 0.68 | 0.130323 |
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Target: 5'- --uUCGCUGCCGUGAUGCUugcgucggguguGCGCAGCa -3' miRNA: 3'- ggcGGCGGUGGCGCUGCGG------------CGUGUUG- -5' |
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| 20958 | 3' | -61.3 | NC_004724.1 | + | 811 | 1.11 | 0.000033 |
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Target: 5'- aCCGCCGCCACCGCGACGCCGCACAACa -3' miRNA: 3'- -GGCGGCGGUGGCGCUGCGGCGUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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