Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21014 | 3' | -50.9 | NC_004735.1 | + | 126709 | 0.66 | 0.990746 |
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Target: 5'- gGCGAcgauGCGGgaUGUCGGUUUUgAcACCg -3' miRNA: 3'- -CGCU----UGCCgaGCAGCUAAAGgUcUGG- -5' |
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| 21014 | 3' | -50.9 | NC_004735.1 | + | 57906 | 0.66 | 0.989021 |
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Target: 5'- cUGAACGGCUUGUCGucg-CCGcguucgaauucuccGAUCa -3' miRNA: 3'- cGCUUGCCGAGCAGCuaaaGGU--------------CUGG- -5' |
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| 21014 | 3' | -50.9 | NC_004735.1 | + | 125979 | 0.67 | 0.984647 |
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Target: 5'- uGCGccgGGCaugcgccaugaUCGUCGAcaggaUUUCCAGAUCg -3' miRNA: 3'- -CGCuugCCG-----------AGCAGCU-----AAAGGUCUGG- -5' |
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| 21014 | 3' | -50.9 | NC_004735.1 | + | 72512 | 0.67 | 0.982728 |
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Target: 5'- aCGAAUGGCUUcgGUCGAUgggUCUcgaaGGuACCg -3' miRNA: 3'- cGCUUGCCGAG--CAGCUAa--AGG----UC-UGG- -5' |
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| 21014 | 3' | -50.9 | NC_004735.1 | + | 126156 | 0.71 | 0.905284 |
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Target: 5'- cCGGACGGCUCcgaagcucaagGUUGAUcUCCGGAauauCCa -3' miRNA: 3'- cGCUUGCCGAG-----------CAGCUAaAGGUCU----GG- -5' |
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| 21014 | 3' | -50.9 | NC_004735.1 | + | 72322 | 0.98 | 0.043242 |
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Target: 5'- aGCGAACGGCUgcUCGAUUUCCAGACCg -3' miRNA: 3'- -CGCUUGCCGAgcAGCUAAAGGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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