miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21048 3' -53.4 NC_004745.1 + 4350 0.66 0.570892
Target:  5'- gUCGGUggUgCUGGCgAUggagccggugacACCGAUGCCAa -3'
miRNA:   3'- aAGUCA--AgGACCG-UA------------UGGCUACGGUc -5'
21048 3' -53.4 NC_004745.1 + 1178 0.67 0.559216
Target:  5'- aUUCAcc-UUUGGCGaGCCGGUGCCGGu -3'
miRNA:   3'- -AAGUcaaGGACCGUaUGGCUACGGUC- -5'
21048 3' -53.4 NC_004745.1 + 16769 0.67 0.513281
Target:  5'- uUUCGuUUCCUcGCGUcGCCGGUGCCGu -3'
miRNA:   3'- -AAGUcAAGGAcCGUA-UGGCUACGGUc -5'
21048 3' -53.4 NC_004745.1 + 16949 1.06 0.00088
Target:  5'- aUUCAGUUCCUGGCAUACCGAUGCCAGu -3'
miRNA:   3'- -AAGUCAAGGACCGUAUGGCUACGGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.