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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2113 | 5' | -41 | NC_001350.1 | + | 36186 | 0.66 | 1 |
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Target: 5'- --aAAGAGaauUUUUAGGGC-UUGAGAGa -3' miRNA: 3'- aaaUUUUC---AAAAUCUCGgAACUCUCg -5' |
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| 2113 | 5' | -41 | NC_001350.1 | + | 30985 | 0.67 | 1 |
|
Target: 5'- ---cAAGGggUUGGAGCaaauguugaucaggUUUGGGAGCu -3' miRNA: 3'- aaauUUUCaaAAUCUCG--------------GAACUCUCG- -5' |
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| 2113 | 5' | -41 | NC_001350.1 | + | 90074 | 0.67 | 1 |
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Target: 5'- cUUUGGGAGUUUUAG-GCUUUcuuGGAGUu -3' miRNA: 3'- -AAAUUUUCAAAAUCuCGGAAc--UCUCG- -5' |
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| 2113 | 5' | -41 | NC_001350.1 | + | 12685 | 0.7 | 0.999975 |
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Target: 5'- aUUGuuAGaugUGGAuGCUUUGAGAGCc -3' miRNA: 3'- aAAUuuUCaaaAUCU-CGGAACUCUCG- -5' |
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| 2113 | 5' | -41 | NC_001350.1 | + | 44973 | 0.74 | 0.998539 |
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Target: 5'- -aUGAAAGU---GGAGUCU-GGGAGCa -3' miRNA: 3'- aaAUUUUCAaaaUCUCGGAaCUCUCG- -5' |
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| 2113 | 5' | -41 | NC_001350.1 | + | 2321 | 0.8 | 0.955123 |
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Target: 5'- ---cAAAGUUUaa-AGCCUUGAGAGCa -3' miRNA: 3'- aaauUUUCAAAaucUCGGAACUCUCG- -5' |
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| 2113 | 5' | -41 | NC_001350.1 | + | 2431 | 1.14 | 0.033043 |
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Target: 5'- gUUUAAAAGUUUUAGAGCCUUGAGAGCa -3' miRNA: 3'- -AAAUUUUCAAAAUCUCGGAACUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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