Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21181 | 3' | -49.6 | NC_004778.3 | + | 121504 | 0.66 | 0.986685 |
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Target: 5'- cGAGGGCGGCCgu-GAGUGgGU-CAu -3' miRNA: 3'- uUUUCCGCCGGaauUUCGCaCAuGUu -5' |
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| 21181 | 3' | -49.6 | NC_004778.3 | + | 96142 | 0.66 | 0.986685 |
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Target: 5'- -uGAGGCGGCUaUucGGCGaccgacUGUGCAAc -3' miRNA: 3'- uuUUCCGCCGGaAuuUCGC------ACAUGUU- -5' |
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| 21181 | 3' | -49.6 | NC_004778.3 | + | 77647 | 0.66 | 0.984851 |
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Target: 5'- uGAAAGGCGacaaGCCccugUUGGGGCG-GUACGAc -3' miRNA: 3'- -UUUUCCGC----CGG----AAUUUCGCaCAUGUU- -5' |
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| 21181 | 3' | -49.6 | NC_004778.3 | + | 89012 | 0.67 | 0.975546 |
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Target: 5'- --cGGGCGGCUcauUUAcuGCGUGUcCAAa -3' miRNA: 3'- uuuUCCGCCGG---AAUuuCGCACAuGUU- -5' |
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| 21181 | 3' | -49.6 | NC_004778.3 | + | 42500 | 0.68 | 0.966228 |
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Target: 5'- uGAGGGCGGCgUggucGGCGUGcccgACAAc -3' miRNA: 3'- uUUUCCGCCGgAauu-UCGCACa---UGUU- -5' |
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| 21181 | 3' | -49.6 | NC_004778.3 | + | 78292 | 0.7 | 0.914251 |
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Target: 5'- cGAAGGCGGCaaaguGcuucagccgcgcgccGGCGUGUACGAa -3' miRNA: 3'- uUUUCCGCCGgaau-U---------------UCGCACAUGUU- -5' |
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| 21181 | 3' | -49.6 | NC_004778.3 | + | 69789 | 0.71 | 0.859474 |
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Target: 5'- cGAGAGGCGGCCgugccucgcgAccGCGUGUcGCGAc -3' miRNA: 3'- -UUUUCCGCCGGaa--------UuuCGCACA-UGUU- -5' |
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| 21181 | 3' | -49.6 | NC_004778.3 | + | 105629 | 0.72 | 0.81477 |
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Target: 5'- --uGGGCGGCCUUu-AGCGcGUGCu- -3' miRNA: 3'- uuuUCCGCCGGAAuuUCGCaCAUGuu -5' |
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| 21181 | 3' | -49.6 | NC_004778.3 | + | 128413 | 1.05 | 0.013455 |
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Target: 5'- gAAAAGGCGGCCUUAAAGCGUGUACAAa -3' miRNA: 3'- -UUUUCCGCCGGAAUUUCGCACAUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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