Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21848 | 3' | -61.5 | NC_004902.1 | + | 6349 | 0.68 | 0.230269 |
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Target: 5'- -cGGUGGUuGCCACCcaGGCuauGGCUCCGg -3' miRNA: 3'- acCCACCGuUGGUGG--CCGu--CUGGGGU- -5' |
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| 21848 | 3' | -61.5 | NC_004902.1 | + | 36661 | 0.69 | 0.21308 |
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Target: 5'- cGGcGUGGCAguaGCUACUGGCGacGGCgCCAc -3' miRNA: 3'- aCC-CACCGU---UGGUGGCCGU--CUGgGGU- -5' |
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| 21848 | 3' | -61.5 | NC_004902.1 | + | 5811 | 0.69 | 0.2076 |
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Target: 5'- gGGGUGGUcaGugCACCaGCAGAgCCg- -3' miRNA: 3'- aCCCACCG--UugGUGGcCGUCUgGGgu -5' |
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| 21848 | 3' | -61.5 | NC_004902.1 | + | 31352 | 0.69 | 0.207059 |
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Target: 5'- aGGGUGGCGgauacaaGCCACCggaGGCAGGUCgCGg -3' miRNA: 3'- aCCCACCGU-------UGGUGG---CCGUCUGGgGU- -5' |
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| 21848 | 3' | -61.5 | NC_004902.1 | + | 6185 | 0.74 | 0.094469 |
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Target: 5'- -aGGUGGUAGCCugCGGgauCAGACCCa- -3' miRNA: 3'- acCCACCGUUGGugGCC---GUCUGGGgu -5' |
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| 21848 | 3' | -61.5 | NC_004902.1 | + | 6409 | 1.07 | 0.000226 |
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Target: 5'- cUGGGUGGCAACCACCGGCAGACCCCAc -3' miRNA: 3'- -ACCCACCGUUGGUGGCCGUCUGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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