miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2192 5' -58.3 NC_001396.1 + 1875 0.66 0.10097
Target:  5'- -aGGCgauGGgGCAGGGGAuGGCCucGCguGCc -3'
miRNA:   3'- uaCCG---UCgCGUUCCUU-CCGG--UGguCG- -5'
2192 5' -58.3 NC_001396.1 + 3817 0.69 0.055135
Target:  5'- -aGGCuGUGCcAGGuuguAGGCCaugACCAGUg -3'
miRNA:   3'- uaCCGuCGCGuUCCu---UCCGG---UGGUCG- -5'
2192 5' -58.3 NC_001396.1 + 5219 0.69 0.048113
Target:  5'- -cGGCuaccGGCGa---GAGGGCCGCUAGCa -3'
miRNA:   3'- uaCCG----UCGCguucCUUCCGGUGGUCG- -5'
2192 5' -58.3 NC_001396.1 + 1450 1.1 0.000013
Target:  5'- aAUGGCAGCGCAAGGAAGGCCACCAGCa -3'
miRNA:   3'- -UACCGUCGCGUUCCUUCCGGUGGUCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.