miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21954 3' -54.5 NC_004914.1 + 44042 0.66 0.768966
Target:  5'- -cAUCuuUGCCagaCCGCGcugGGUAAGCGUu -3'
miRNA:   3'- aaUAGcgACGGg--GGCGC---UUAUUCGCG- -5'
21954 3' -54.5 NC_004914.1 + 48495 0.68 0.650365
Target:  5'- -cGUCGUcgGCCUCaguuaGCcAGUGAGCGCa -3'
miRNA:   3'- aaUAGCGa-CGGGGg----CGcUUAUUCGCG- -5'
21954 3' -54.5 NC_004914.1 + 7359 0.7 0.540137
Target:  5'- --uUCGCUgGCCCCUGCagGAAUu-GUGCg -3'
miRNA:   3'- aauAGCGA-CGGGGGCG--CUUAuuCGCG- -5'
21954 3' -54.5 NC_004914.1 + 60385 0.71 0.487216
Target:  5'- aUcgCGCUGCgCUuuGUaGGUGAGCGCg -3'
miRNA:   3'- aAuaGCGACG-GGggCGcUUAUUCGCG- -5'
21954 3' -54.5 NC_004914.1 + 3323 0.73 0.362972
Target:  5'- ---aCGCUGCCCCUGUGucaGGGCGa -3'
miRNA:   3'- aauaGCGACGGGGGCGCuuaUUCGCg -5'
21954 3' -54.5 NC_004914.1 + 55795 0.8 0.122474
Target:  5'- cUGUCGaugaUGCCCCCGCGuuu-AGCGCc -3'
miRNA:   3'- aAUAGCg---ACGGGGGCGCuuauUCGCG- -5'
21954 3' -54.5 NC_004914.1 + 3559 1.1 0.001007
Target:  5'- cUUAUCGCUGCCCCCGCGAAUAAGCGCa -3'
miRNA:   3'- -AAUAGCGACGGGGGCGCUUAUUCGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.