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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21986 | 3' | -49.6 | NC_004914.1 | + | 37085 | 0.66 | 0.971427 |
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Target: 5'- aGGGAgcuACAGGCUccuuuuuuauUAUucgCAUUCACCCu -3' miRNA: 3'- -CCUUau-UGUCCGA----------AUG---GUAGGUGGGc -5' |
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| 21986 | 3' | -49.6 | NC_004914.1 | + | 17173 | 0.66 | 0.964672 |
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Target: 5'- cGggUGGgAGGcCUUGCCAcuUCCAgucUCCGu -3' miRNA: 3'- cCuuAUUgUCC-GAAUGGU--AGGU---GGGC- -5' |
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| 21986 | 3' | -49.6 | NC_004914.1 | + | 3572 | 0.68 | 0.914354 |
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Target: 5'- uGAGUAAauaAGGCUUAUCG-CUGCCCc -3' miRNA: 3'- cCUUAUUg--UCCGAAUGGUaGGUGGGc -5' |
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| 21986 | 3' | -49.6 | NC_004914.1 | + | 16403 | 0.69 | 0.878404 |
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Target: 5'- aGggUGGCAGGaUUACaaaaauauccUCCGCCCGg -3' miRNA: 3'- cCuuAUUGUCCgAAUGgu--------AGGUGGGC- -5' |
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| 21986 | 3' | -49.6 | NC_004914.1 | + | 3878 | 0.71 | 0.766941 |
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Target: 5'- gGGAAUGACGuGUUUACCGUCgcgggugagCACCCc -3' miRNA: 3'- -CCUUAUUGUcCGAAUGGUAG---------GUGGGc -5' |
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| 21986 | 3' | -49.6 | NC_004914.1 | + | 58940 | 1.13 | 0.002415 |
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Target: 5'- aGGAAUAACAGGCUUACCAUCCACCCGg -3' miRNA: 3'- -CCUUAUUGUCCGAAUGGUAGGUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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