Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22005 | 5' | -57.8 | NC_004914.1 | + | 21502 | 0.66 | 0.591207 |
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Target: 5'- cGUGAaggGCuACCGGCUCCUGACauUGcGCGu -3' miRNA: 3'- -UAUUa--CGuUGGCCGGGGGCUG--AC-CGU- -5' |
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| 22005 | 5' | -57.8 | NC_004914.1 | + | 15321 | 0.66 | 0.58038 |
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Target: 5'- -gGAUGUGGcuuacgaagguuCCGGCUCUggCGACUGGCGc -3' miRNA: 3'- uaUUACGUU------------GGCCGGGG--GCUGACCGU- -5' |
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| 22005 | 5' | -57.8 | NC_004914.1 | + | 6189 | 0.66 | 0.548185 |
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Target: 5'- ---cUGCAACCGGCaCCCUGAauguuCUGcuGCGu -3' miRNA: 3'- uauuACGUUGGCCG-GGGGCU-----GAC--CGU- -5' |
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| 22005 | 5' | -57.8 | NC_004914.1 | + | 9032 | 0.68 | 0.454782 |
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Target: 5'- cGUGggGaGACUGGCCCCCGAggugaacCUGGUc -3' miRNA: 3'- -UAUuaCgUUGGCCGGGGGCU-------GACCGu -5' |
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| 22005 | 5' | -57.8 | NC_004914.1 | + | 19714 | 0.68 | 0.436358 |
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Target: 5'- -gGAUGCAGCCGGaaagagCCaCgGcACUGGCAg -3' miRNA: 3'- uaUUACGUUGGCCg-----GG-GgC-UGACCGU- -5' |
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| 22005 | 5' | -57.8 | NC_004914.1 | + | 58115 | 0.7 | 0.363801 |
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Target: 5'- ---uUGCuuCCGGCUgaCUGACUGGCGc -3' miRNA: 3'- uauuACGuuGGCCGGg-GGCUGACCGU- -5' |
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| 22005 | 5' | -57.8 | NC_004914.1 | + | 12770 | 0.7 | 0.355338 |
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Target: 5'- uUAcgGCGAuCCGGUugaCCCUGGCUGGUc -3' miRNA: 3'- uAUuaCGUU-GGCCG---GGGGCUGACCGu -5' |
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| 22005 | 5' | -57.8 | NC_004914.1 | + | 16744 | 0.71 | 0.292703 |
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Target: 5'- ---cUGCGauACCuGCCCCCGugUGGUu -3' miRNA: 3'- uauuACGU--UGGcCGGGGGCugACCGu -5' |
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| 22005 | 5' | -57.8 | NC_004914.1 | + | 60546 | 1.07 | 0.000792 |
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Target: 5'- gAUAAUGCAACCGGCCCCCGACUGGCAg -3' miRNA: 3'- -UAUUACGUUGGCCGGGGGCUGACCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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